Whenever you ask for help from FlowJo, it launches a web browser and accesses help pages about the topic you selected.
FLOWJO 10 LAYOUT EDITOR HOW TO
You can learn more about FlowJo, and in particular how to use these platforms, through the on-line help facility. The tutorial is only an introduction! FlowJo is capable of much that simply cant be covered in an introduction such as this (for example, there are Analysis Platforms to perform sophisticated DNA/Cell Cycle analysis, Kinetics analysis, Compensation, etc). The steps shown in this tutorial will help you in the analysis of nearly any kind of FACS data. The experiments used for the tutorial are based on 3-color immunophenotyping of human peripheral blood mononuclear cells (PBMC). The tutorial is designed around an example data set. It will take you three to six hours to complete the tutorial (you can easily break it up by chapter). As you watch FlowJo perform various operations such as creating new graphs, statistics, tables, or graphical layouts, you will see how fast and easy FlowJo is to use. Run the program as you perform the steps in the tutorial so that you can get the best feel for how the program works. Reading through it, you will learn how to operate FlowJo. This tutorial is designed to introduce you to the program.
The Workspace is saved as a FlowJo document on your hard disk when you re-open the document, you will see the status of your analyses as they were when you last saved the Workspace. The Workspace contains a list of all of the data samples that you load, the gates, statistics and other analyses that you apply, and the table templates and graphical layout templates that you design. This environment is presented in the form of a Workspace.
FLOWJO 10 LAYOUT EDITOR SOFTWARE
FlowJo Advanced Tutorial MMXRevision Date: FebruVersion 7.6įlowJo TutorialFlowJo is a software application designed to create an integrated environment for viewing and analyzing flow cytometric data. FlowJo, its tutorials, documentation and web site are Copyright Tree Star, Inc. We are indebted to our active and enthusiastic users worldwide for their ideas, discussions and tireless testing of new versions.
Data Analysis Software for Flow CytometryAdvanced TutorialįlowJo was written by Adam Treister and Mario Roederer beginning in 1996, based on concepts developed at the Herzenberg laboratory at Stanford.
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